1. Immobilized human PCSK9 at 10 g/ml (100 l/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.61 g/ml. 2. Immobilized mouse PCSK9 at 10 g/ml (100 l/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 g/ml.
Properties
Purity
> 85 % as determined by reducing SDS-PAGE.
Endotoxin
< 1.0 EU per g of the protein as determined by the LAL method.
Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Formulation
Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution
Please refer to the printed manual for detailed information.
Background LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.
Express delivery and return policies
Free shipping on orders over $30
Processing time: 1-2 Days
Shipping time: 3-5 Days(According to the logistics method and delivery address)
Items can be returned or exchanged within 30 days !
